Skip to main content
. Author manuscript; available in PMC: 2014 Apr 29.
Published in final edited form as: Methods Enzymol. 2012;517:89–109. doi: 10.1016/B978-0-12-404634-4.00005-X

Figure 4.

Figure 4

EDTA competition assay to determine the pFe for amychelin. Fe-amychelin is incubated with various [EDTA]. Increasing EDTA concentrations result in disappearance of the Fe-amychelin charge transfer band, indicating conversion of Fe-amychelin into apo-amychelin and Fe-EDTA. The data can be deconvoluted as described in Section 4 to obtain a pFe for amychelin.