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. Author manuscript; available in PMC: 2014 Apr 29.
Published in final edited form as: Cancer Res. 2011 Oct 31;71(24):7597–7607. doi: 10.1158/0008-5472.CAN-11-1080

Figure 4.

Figure 4

Inhibition of IGF-1R/InsR, ERK/Akt phospho signaling pathways by BMS-754807 alone or combination with 4-OH Tam (10 μmol/L), letrozole (10 μmol/L), or fulvestrant (100 nmol/L). Briefly, cells were cultured in IMEM steroid–reduced medium without phenol red with 1 nmol/L androstenedione for 24 hours, then subconfluent MCF-7/AC-1 cells were treated with either DMSO, BMS-754807 at the indicated concentrations, or BMS-754807 plus hormones for 24 hours in serum-free conditions. For the final 15 minutes of drug treatment, 10 nmol/L LongR3 IGF-I was added to the medium. Lysates were then prepared and analyzed by Western blotting, as described in the Methods.

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