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. Author manuscript; available in PMC: 2015 Apr 25.
Published in final edited form as: J Chromatogr A. 2014 Mar 3;1339:73–79. doi: 10.1016/j.chroma.2014.02.074

Figure 5. The linkage of rEP18 to hydrazide-agarose is stable for more than a month.

Figure 5

A 50 μl bed column was prepared by coupling 50 μl of 200 nM radiolabeled rEP18 using NaIO4 and Na2S2O5 as described and coupled to hydrazide-agarose for 4h. The column was extensively washed with TE0.1–TE2.0. The column was counted at various times by Cerenkov radiation. Circles show the column after washing and the triangles show the column after its use for affinity chromatography. The experiment was repeated four independent times with similar results.