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. Author manuscript; available in PMC: 2015 Apr 25.
Published in final edited form as: J Chromatogr A. 2014 Mar 3;1339:73–79. doi: 10.1016/j.chroma.2014.02.074

Figure 6. Affinity chromatography of GFP-C/EBP from crude bacterial extract.

Figure 6

A 50 μl column prepared from 100 nM rEP18 was used to purify the GFP-C/EBP from a crude bacterial extract. Elution was as described for Fig. 4. A, Average epifluorescent measurements from two independent experiments are shown ± standard deviation. B, samples were concentrated, applied to 12% SDS-PAGE, and stained with silver. P, GFP-C/EBP purified by Ni2+-agarose affinity chromatography; C, crude bacterial extract; F, flow-through (unretained) fraction; W1–W10, TE0.1 washes.; -, no sample applied.