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. Author manuscript; available in PMC: 2015 Mar 20.
Published in final edited form as: Mol Cell. 2014 Mar 20;53(6):929–940. doi: 10.1016/j.molcel.2014.02.029

Figure 2. Reconstitution of AgrC-I dimers into nanodiscs, see also Figure S2.

Figure 2

(A) Schematic showing the workflow used for reconstituting AgrC-I dimers into nanodiscs. (B) Purification of reconstituted AgrC-I discs by Ni-NTA affinity chromatography as analyzed by SDS-PAGE with Coomassie-brilliant blue (CBB) staining. (C) AgrC-I discs analyzed by SEC. Fractions collected between the dashed lines were further analyzed by SDS-PAGE with CBB staining (bottom). (D) SEC-MALS analysis of empty and purified AgrC-I discs. Moverall, average molecular weight (Mw) of the peak; Mprotein, Mw of proteins in each case; AgrC-I = 51 kDa, MSP = 29 kDa. (E) Stoichiometry of AgrC-I discs as analyzed by SDS-PAGE with CBB staining: AgrC-I discs (left lane) and an equimolar mixture of purified AgrC-I and MSP (right lane).