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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Cancer Immunol Res. 2013 Dec 17;2(4):341–350. doi: 10.1158/2326-6066.CIR-13-0138

Figure 2.

Figure 2

Determination of minimum epitopes for TR-CD4 and NTR-CD4. (A-C) Dose-dependence of NY-ESO-1 peptide recognition by TR-CD4 and NTR-CD4. Indicated peptides were pulsed overnight on SK29 at indicated concentrations. Recognition by TR-CD4 and NTR-CD4 was evaluated by IFN-γ ELISPOT assays. (D) Dose-dependence of NY-ESO-1 protein recognition. NY-ESO-1 protein was pulsed on DP04+ immature monocyte-derived DCs overnight at indicated concentrations. TR-CD4 and NTR-CD4 were stimulated with these DCs for 24 hours. IFN-γ production in the supernatant was measured by ELISA. (E) Recognition of overlapping NY-ESO-1 peptide-pulsed SK29 was evaluated by intracellular IFN-γ-staining. (F) Dose-dependence of NY-ESO-1161–169 recognition. NY-ESO-1161–169 was pulsed on SK29 at indicated concentrations. Recognition was evaluated by intracellular IFN-γ staining. All experiments were repeated at least twice with consistent results. Error bars indicate s.d. from duplicated wells.

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