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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Cancer Immunol Res. 2013 Dec 17;2(4):341–350. doi: 10.1158/2326-6066.CIR-13-0138

Figure 5.

Figure 5

Effect of inhibitors for MHC-I antigen-processing pathways on the recognition of SK37 by TR-CD4. (A) SK37 was treated by the indicated inhibitors for 16–20 hours followed by fixing with paraformaldehyde and extensive washes. SK37 was co-cultured for 20 hours with TR-CD4. IFN-γ level in the supernatant was measured by ELISA. (B) Role of TAP in the presentation. Untreated parental SK37 and SK37 clones stably expressing ICP47 gene were used as stimulator cells. GM-CSF level in the supernatant was measured by ELISA. Statistical significance was calculated by Student’s t-test and is shown as *:P ≤ 0.05; **:P ≤ 0.01; and *** P ≤ 0.001.

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