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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Cancer Immunol Res. 2013 Dec 17;2(4):341–350. doi: 10.1158/2326-6066.CIR-13-0138

Figure 6.

Figure 6

Effect of inhibitors for previously characterized endogenous MHC-II presentation pathways on the recognition of SK37 by TR-CD4. SK37 was treated by the indicated inhibitors for 40–44 hours (A, C) or 16–20 hours (D) followed by fixing with paraformaldehyde and extensive washes. SK37 was co-cultured for 20 hours with TR-CD4. GM-CSF level in the supernatant was measured by ELISA. (A) Effect of an inhibitor for macroautophagy. (B) Effect of siRNA-mediated silencing of LAMP2. SK37 was electroporated with indicated siRNA and cultured for 3 days. (C) Effect of an endosomal/lysosomal recycling inhibitor. (D) Effect of vesicular transport and protein synthesis inhibitors. All experiments were repeated at least three times with consistent results. Error bars indicate s.d. from duplicated wells. Statistical significance was calculated by Student’s t-test and is shown as *:P ≤ 0.05 and **:P ≤ 0.01.

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