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. 2014 Mar 14;15(1):195. doi: 10.1186/1471-2164-15-195

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© Flagel et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Rarefaction curve of SNP discovery among the 26 WCR populations sampled. Starting with the first population sampled and continuing until the 26^th we record the number of new SNPs each population contributes. The slope of the rarefaction curve is dependent on population input order, which in this case is arbitrary. To account for this, we selected 100 random input orders and computed the mean number of new SNPs discovered (solid black line with filled points) and the upper and lower 95% confidence intervals (dashed grey lines).