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. 2014 Mar 19;15(1):32. doi: 10.1186/1465-9921-15-32

Figure 11.

Figure 11

Activation of PKC is required for Vegf-mediated anti-apoptotic responses. A-B. E11.5 lung explants exposed to either 3% oxygen or 50% oxygen ± Vegf165 (100 ng/ml) ± GO6983 (5 μM) for 3 days and stained for TUNEL, cytokeratin and DAPI. (A) Quantitation of total TUNEL positive cells/total cells x 100 in explants cultured. (B) Quantitation of total TUNEL positive, cytokeratin positive cells/total cytokeratin positive cells. *p <0.05 vs. 50% oxygen, 50% oxygen + GO6983 and 50% oxygen + GO6983 + Vegf165 at E11.5 + 3. C-D E11.5 lung explants exposed to either 3% oxygen or 50% oxygen ± Vegf165 (100 ng/ml) ± GO6983 (5 μM) for 3 days and stained for cleaved caspase 3, cytokeratin and DAPI. (C) Quantitation of total cleaved caspase 3 positive cells/total cells x 100. (D) Quantitation of total cleaved caspase 3 positive, cytokeratin positive cells/total cytokeratin positive cells. *p <0.05 vs. 50% oxygen, 50% oxygen + GO6983 and 50% oxygen + GO6983 + Vegf165 at E11.5 + 3. E-F E15.5 Post caval lung explants exposed to either 3% oxygen or 50% oxygen ± Vegf165 (100 ng/ml) ± GO6983 (5 μM) for 2 days and stained for cleaved caspase 3, cytokeratin and DAPI. (E) Quantitation of total cleaved caspase 3 positive cells/total cells x 100. (F) Quantitation of total cleaved caspase 3 positive, cytokeratin positive cells/total cytokeratin positive cells. *p <0.05 vs. 50% oxygen, 50% oxygen + GO6983 and 50% oxygen + GO6983 + Vegf165 at E15.5 + 2 .n = 3–4 separate experiments with 2–3 lungs/ condition/experiment.