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Hyperoxia suppresses endothelial cell apoptosis. FLK1-eGFP E11.5 lungs were harvested and exposed for 2 d. to (A) 3% oxygen, (B) 50% oxygen + Isotype, (C) 50% oxygen + Vegf165 (100 ng/ml). (A’) Quantitation of TUNEL positive, GFP positive cells/total GFP positive cells. ***p <0.05 vs. 50% + Isotype and 50% + Vegf165. (B’) Total number of GFP positive (endothelial) cells and GFP negative (non-endothelial) cells counted for this experiment in thousands during exposure to 3% oxygen.
