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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Nat Neurosci. 2013 Dec 15;17(2):269–279. doi: 10.1038/nn.3614

Figure 3. Longitudinal projections of Dentate mossy fibers (MFs) within CA2.

Figure 3

a, Schematic of DGGC-specific transgenic Cre mouse, Cre-dependent tTA lentivirus and tTA-dependent ArchT-GFP-AAV9. Cre-recombinase switches on the expression of tTA in DGGCs inducing the expression of ArchT-GFP in DGGCs. b, Dorsorostral injection-site (green) and location of serial sections in (c). c, Serial images of RGS14, ArchT-GFP and synaptoporin (MF marker). DG cells in sections #1–3 express ArchT-GFP. d, Confocal image from the dotted line-box in (c). e, Longitudinal projections of MFs in CA2. f–h, Same approach applied to dorsal (f), intermediate (g) and ventral (h) DG injection sites (green dots) and location of serial sections in (i, j, k) respectively. i–k, Images showing extent and direction of MFs (green) within the RGS14-positive (red) CA2 region for dorsal (i), intermediate (j) and ventral (k) DG injection sites. l–n, Magnified confocal images from the corresponding dotted line-boxes in (i, j, k). o–q, Normalized fluorescence intesity of GFP-positive MFs terminals within dorsal (D), intermediate (I) and ventral (V) CA2, for dorsal (o), intermediate (p) and ventral (q) DG injection sites (n=6 samples per group from n=3 mice per injection site). Data are represented as mean ± SEM.