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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Nat Neurosci. 2013 Dec 15;17(2):269–279. doi: 10.1038/nn.3614

Figure 4. Functional monosynaptic connection of MF terminals onto CA2 pyramidal cells.

Figure 4

a, DGGC-specific Cre transgenic mice were injected with Cre-dependent AAV9-EF1α-ChR2-YFP. Cre-recombinase switches on the expression of ChR2-YFP in DGGCs. b, DGGC-specific expression of ChR2-YFP (top) and enlarged image from the dotted line-box (bottom). c, Optogenetic stimulation of ChR2-YFP-positive DGGCs. d, Zeta-projected confocal image of a recorded CA2 pyramidal cell (yellow) overlapped by ChR2-YFP-positive MFs (green). Bottom: single confocal stacks showing colocalization of biocytin (red) and RGS14 (cyan). e, Patch-clamp recording of CA2 pyramidal cell and optogenetic stimulation of MFs in acute hippocampal slice. f–g, Optogenetic stimulation of MFs elicited an EPSC in a CA2 pyramidal cell (f) which is sensitive to NBQX/AP5 (n=5, two tailed paired t-test: *P<0.05) and DCG-IV (n=5, two tailed paired t-test: *P<0.05) (g). Data are represented as mean ± SEM. h, Representative zeta-projected confocal image of CA1, CA2 and CA3 pyramidal cells recorded in the same slice. i–j, EPSCs recorded in CA1, CA2 and CA3 pyramidal cells elicited by optogenetic stimulation of MFs (i). CA2 and CA3 cells displayed a fast EPSC onset whereas CA1 cells displayed a delayed onset (j, n=9 triplets, for CA1 and CA2, two tailed paired: t-test ***P<0.001, for CA3 and CA2 *P<0.05).