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. 2013 Dec 30;35(5):1100–1109. doi: 10.1093/carcin/bgt489

Fig. 3.

Fig. 3.

EDD regulates Mcl-1 levels through transcriptional regulation. (A) EDD knockdown inhibits Mcl-1 mRNA expression. ES-2 and A2780ip2 cells were transfected with EDD siRNA1 for 24 or 12h, respectively, and RNA was harvested. Quantitative real-time PCR was performed using Mcl-1-specific primers. The y-axis represents the fold change in Mcl-1 mRNA in EDD siRNA1-transfected cells compared with that in control siRNA-transfected cells. The results are a combination of three independent experiments. (B) EDD activates the Mcl-1 promoter. HeLa cells were transfected with p(−2389/+10)mcl-luc, an Mcl-1 promoter-driven firefly luciferase plasmid (16), TK Renilla luciferase and either Flag-EDD, Flag-EDD-C2768A or empty vector. Cells were harvested at 48h and firefly luciferase activity was normalized to Renilla luciferase activity in each sample. P values indicate significance (P < 0.05) within a group between Flag-EDD- and vector-transfected cells. These results are a combination of four independent experiments. (C) Western blot of Flag-EDD from (B).