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In-gel fluorescence analysis showed that most CD38 was present on plasma membrane. Lanes 1, live cell labeling with Rh-6-(F-araNAD) followed by in-gel fluorescence (labeling plasma membrane CD38 only); lanes 2, whole cell lysate was obtained first followed by labeling with Rh-6-(F-araNAD) (labeling all catalytically active CD38); lanes 3, whole cell lysate without CD38 probes. Protein ladder is on the left, listed first. The full gel images are shown in Figure S7 in SI.
