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Dissociation constant measurements. Electrophoeretic mobility shift assay (EMSA) of 32P-labeled 3WJb (constant concentration) assembling with increasing concentrations of 3WJa and 3WJc for RNA, 2′-F RNA, and DNA on 12% polyacrylamide gels in native conditions. Plot of percentage of 3WJ formed versus total concentration to solve for KD of each 3WJ.
