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Assembly curves produced from the Roche 480 Lightcycler using SYBR green II reporter dye. (A) 3WJRNA, (B) 3WJDNA, (C) 3WJ2′-F, and each of the components of the 3WJs. (D) The three 3WJ species directly compared, showing the differences in annealing temperatures (Ta).
