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Representative 15% native TGGE with temperature gradient perpendicular to the electrical current. (A) 3WJ2′-F, (B) 3WJRNA, and (C) 3WJDNA; total 3WJ concentration in each lane = 10 μM. Panels (D) and (E) are control gels showing migration of 3WJRNA (D) before reaching its Tm in the temperature range of 20–40 °C and migration of 3WJDNA (E) in the temperature range of 40–70 °C that is over its Tm. The 3WJ bands were detected by total nucleic acid stain with EB.
