Table 1.
Test | Principle | Specimen volume required | Antigen employed | Time to test result | Specimen type | Sensitivity | Specificity |
---|---|---|---|---|---|---|---|
SD Bioline HIV-1/2 3.0 | Lateral flow immunochromatography | 10 μL | gp 41 and p24 for HIV-1 and gp 36 for HIV-2 | 5–20 minutes |
Serum, plasma |
Not specified |
Not specified |
Pareekshak HIV-1/2 Triline card test | Lateral flow immunochromatography | 10 μL | gp 41 and C terminus of gp 120 for HIV-1 and gp 36 for HIV-2 | 20 minutes |
Serum, plasma |
100% | 99.49% |
Pareekshak HIV 1/2 rapid test kit (Trispot) | Immunoconcentration | 2 drops | HIV 1 and HIV-2 r (recombinant)-proteins | Immediate | Serum, plasma |
100% | 100% |
Microlisa-HIV | Indirect ELISA | 10–20 μL | gp 41 and C terminus of gp 120 for HIV-1 and gp 36 for HIV-2 | >1.5 hours |
Serum, plasma |
100% | 99.5% |
HIV: human immunodeficiency virus; ELISA: enzyme linked immunosorbent assay.