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(A) Selection conditions and percentage of radioactivity (counts per min) in eluted fractions. Concentrations of the 2G12 listed for the selection are prior to addition of protein G or protein A magnetic beads. (B) Profiling of the distribution of the putative number of glycans in library peptides before selection (“n” on the right on the gel). Red boxes indicate enrichment of low-valent glycopeptides in 37 °C selection rounds.
