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Double-mutant cycle analysis designed to test the collective role of the catalytic base loop in positioning the general base in the KSI active site. (A) Effects on kcat for mutation of the residues in the loop to glycine, where the Gly loop designation refers to the P39G/V40G/S42G/E43G/P44G mutant. (B) Effects on kcat for mutation of the Asp38 general base to Glu with a WT loop sequence and with the residues in the loop mutated to glycine. Values are from Table 7.
