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Double-mutant cycles designed to test the role of the loop residues Pro39–Pro44 in positioning the general base. Effects on kcat for the mutation of loop residues in KSI with a positioned Asp38 general base and a mispositioned D38E mutant general base are shown. Values are plotted relative to WT KSI on the left-hand side and relative to the D38E mutant on the right-hand side. Data are from Table 4.
