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. 2014 Mar 26;53(14):2318–2328. doi: 10.1021/bi401692v

Table 1. Kinetic Parameters for gpASNase3 Hydrolysis of l-Asn and β-l-Asp-l-Phe.

substrate kcat (s–1) KM (mM) kcat/KM (mM–1 s–1)
l-Asn 3.95 ± 0.08 2.24 ± 0.14 1.76 ± 0.15
β-l-Asp-l-Phe 1.98 ± 0.04 0.41 ± 0.03 4.83 ± 0.45