Table 1. Kinetic Parameters for gpASNase3 Hydrolysis of l-Asn and β-l-Asp-l-Phe.
substrate | kcat (s–1) | KM (mM) | kcat/KM (mM–1 s–1) |
---|---|---|---|
l-Asn | 3.95 ± 0.08 | 2.24 ± 0.14 | 1.76 ± 0.15 |
β-l-Asp-l-Phe | 1.98 ± 0.04 | 0.41 ± 0.03 | 4.83 ± 0.45 |
substrate | kcat (s–1) | KM (mM) | kcat/KM (mM–1 s–1) |
---|---|---|---|
l-Asn | 3.95 ± 0.08 | 2.24 ± 0.14 | 1.76 ± 0.15 |
β-l-Asp-l-Phe | 1.98 ± 0.04 | 0.41 ± 0.03 | 4.83 ± 0.45 |