Figure 2.

Sequences of peptides examined in this study. Top: the alanine-rich model α-helical peptide. Middle: peptides with modifications at the N-terminal residue (residue 1), at a central residue (residue 5), and at the C-terminal residue (residue 14; numbering based on other peptides, using the first Ala as residue 1, excluding the N-terminal YG) of a model α-helix. GY or YG sequences were added for concentration determination. The peptide with the C-terminal modifications employed the YG at the N-terminus to avoid potential interaction of the post-translational modification with the tyrosine. Bottom: peptides examined for the effects of phosphorylation at residues 2 and 10. Peptides were also synthesized with proline in place of serine or threonine at residues 1, 2, 5, 10, and 14.