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CD spectra of Ac-AKAAAAKAATAKAAGY-NH2 peptides with threonine modifications [unmodified Thr (free hydroxyl), ThrOPO3H– (pH 4), and ThrOPO32– (pH 8)]: unmodified Thr (green squares), dianionic phosphothreonine (pH 8) (red circles), and monoanionic phosphothreonine (pH 4) (open magenta circles). CD experiments were conducted in water with 10 mM phosphate (pH 7 or as indicated) and 25 mM KF at 0.5 °C.
