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. 2014 Mar 5;8(4):3192–3201. doi: 10.1021/nn406302j

Figure 3.

Figure 3

Loading of magnetic nanoparticle into lysosomes in INS-1 cells. (A) Confocal imaging of SPIONs location in INS-1 cells. The SPIONs conjugated with the fluorescent dye TRITC (orange) were incubated with living cells in a static magnetic field for 5 min. Thereafter, the cells were treated by DMF with 20 Hz, for 20 min, and then confocal microscopy images were obtained. Plasma membrane and early endosomes were stained with CellMask (red); nuclei and lysosomes were stained with Hoechst 32580 (blue) and LysoTracker Green (green), respectively. The squares in the merge stack indicate SPIONs located in the lysosomes. Scale bars = 2 μm. (B) Statistical analysis of SPION colocalization with LysoTracker Green and CellMask under same conditions as in (A). (C) Colocalization analysis of lysosomes with SPION and CellMask under same conditions as (A). (D) Loading efficiency of LAMP1 antibody conjugated SPIONs (LAMP1-SPION) increased under condition with the DMF treatment. The loading efficiency is calculated by the ratio of TRITC fluorescence intensity (yellow) over nuclear intensity. The data was collected from three independent experiments. *p < 0.05 and ***p < 0.001.