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. 2014 Mar 21;8(4):3444–3450. doi: 10.1021/nn406339n

Figure 3.

Figure 3

Modulation of miRNA·probe blocking level by PEGs of different lengths. (a) PEG length-dependent blocking level (I/I0) for stage Ib of the signatures (as illustrated in Figure 2b) for four miRNAs: miR-155 (●), miR-182-5p (■), miR-210 (▲), miR-21 (▼). The four colored arrows mark an optimized miRNA/probe combination: miR-155·P0 (red), miR-182-5p·P3 (green), miR-210·P8 (blue), and miR-21·P24 (purple). The four miRNA·probe hybrids demonstrate maximally separated blocking levels, enabling accurate detection of multiplex miRNA species. (b) Distinct signatures for the four optimized miRNA·probe hybrids (miR-155·P0, miR-182-5p·P3, miR-210·P8, and miR-21·P24) marked in panel a. (c) Duration of four miRNA·probe signatures with untagged (black bar) and barcoded (gray bar) probes. Melting temperatures of the four miRNAs are also shown (red circles). The block duration is positively correlated to the miRNA·probe melting temperature. Method for obtaining block duration is described in S1 in the SI.