Table 4.

Assignment of TSDs flanking transposed ^a Tos17 in ttm2 and ttm5 to the genome of the original line, Nipponbare

Detected by		Line	Chromosome	b Position of junction for		Size of TSD	TSD for		Direction	Confirmed by PCR/Sequencing
				Tos17 tail	Tos17 head		Tos17 tail	Tos17 head
TIF	RelocaTE	ttm2	chr04	30 259 052	30 259 056	5	GTTTC	GTTTC	Forward	Yes
TIF	RelocaTE	ttm2	chr05	1 925 905	1 925 909	5	CTATC	CTATC	Forward	Yes
TIF	RelocaTE	ttm2	chr10	22 134 718	22 134 714	5	CTTGC	CTTGC	Reverse	Yes
TIF	RelocaTE	ttm2	chr10	22 531 003	22 531 007	5	ACTTT	ACTTT	Forward	Yes
TIF		ttm5	chr01	34 453 645	34 453 641	5	CTTTG	CTTTG	Reverse	Yes
TIF	RelocaTE	ttm5	chr02	1 004 769	1 004 765	5	ATACC	ATACC	Reverse	Yes
TIF	RelocaTE	ttm5	chr02	31 596 628	31 596 632	5	CTAAT	CTAAT	Forward	Yes
TIF	RelocaTE	ttm5	chr03	741 226	741 222	5	GCTGC	GCTGC	Reverse	Yes
TIF	RelocaTE	ttm5	chr03	8 304 678	8 304 674	5	GAATA	GAATA	Reverse	Yes
TIF		ttm5	chr06	24 967 881	24 967 877	5	TGCAT	TGCAT	Reverse	Yes
TIF		ttm5	chr07	20 064 391	20 064 395	5	CTTAT	CTTAT	Forward	cYes
				20 080 552	20 080 556
TIF	RelocaTE	ttm5	chr09	12 970 618	12 970 614	5	CATGC	CATGC	Reverse	Yes
	RelocaTE	ttm5	chr10	14 739 090	14 739 094	5		GAACT	Forward	No
TIF		ttm5	chr10	19 069 885	19 069 889	5	ACTTG	ACTTG	Forward	Yes
TIF	RelocaTE	ttm5	chr10	21 583 054	21 583 058	5	CTTAT	CTTAT	Forward	Yes
TIF	RelocaTE	ttm5	chr12	2 155 899	2 155 895	5	GGAAC	GGAAC	Reverse	Yes

^aOriginal Tos17s are located from 26 694 799 to 26 698 904 at chromosome 7 and from 15 415 378 to 15 419 573 at chromosome 10.

^bpositions of 1^st base of flanking sequences to the tail and head sequence of Tos17.

^cTSD on chromosome 7 in ttm5 is located within one of two very similar sequences; thus, it was difficult to determine its position conclusively from the short read sequences.