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. 2014 Mar 14;14:64. doi: 10.1186/1471-2229-14-64

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Copyright © 2014 Egidi et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Comparison between the 2D electrophoretic patterns of glutenin subunits of the B1133-T23N transgenic lines. A) null segregant control line; B) genotype expressing the B1133-T23N gene; C) Immunoblotting performed by using the anti-FLAG antibody on the gel region indicated by the box in B). Arrows point at the four proteins (spots nr. 5-8) that are absent in the control line. Molecular weight standards (in kDa) are reported on the left side, and the pI range is indicated at the top of the gels.