Table 1.
Comparison between the expected and observed N-terminal amino acid sequences of the eight transgenic proteins
| Genotype | Spot n. | Expected N-terminal sequence | Observed N-terminal sequence |
|---|---|---|---|
|
42K-N23T |
1 |
METSHIPSLEKPLQQ- |
METSHIP- |
|
B1133-WT |
3 |
METSHIFGLERPWQQ- |
METSHIFGLERPWQQ- |
| |
4 |
METSHIFGLERPWQQ- |
METSHIFGLERPWQ- |
|
B1133-T23N |
5 |
SCIPGLERPWQQQPL- |
SCIPGLERP- |
| |
6 |
SCIPGLERPWQQQPL- |
SCIPGLERP- |
| |
7 |
SCIPGLERPWQQQPL- |
SCIPGLERPW- |
| 8 | SCIPGLERPWQQQPL- | SCIPGLERP- |
In the “Genotype” column the three transgenic genotypes here analysed are reported. The “Expected N-terminal sequence” column reports the deduced N-terminal amino acid sequences on the basis of transgene nucleotide sequences, whereas the “Observed N-terminal sequence” corresponds to those obtained after Edman degradation, relatively to each of the eight protein spots indicated in the second column.