TABLE 3.
Abundance of DHFR ori-β target DNA sequences in nascent DNAa
| DNA sourceb | Cell linec | Abundanced of DHFR ori-β target DNA (initiation activitye) with primer pair:
|
|||
|---|---|---|---|---|---|
| 8 | 2 | 6 | 3 | ||
| pMCDf | DR12 | 1.13 × 10−6 (3.7) | 9.10 × 10−6 (29.6) | 2.00 × 10−6 (6.5) | 3.08 × 10−7 (1) |
| pMCDΔATf | DR12 | 1.98 × 10−7 (0.7) | 1.18 × 10−6 (4.3) | 4.45 × 10−7 (1.6) | 2.73 × 10−7 (1) |
| pATrep | DR12 | 1.43 × 10−8 (0.9) | 7.57 × 10−8 (4.5) | 4.29 × 10−8 (2.6) | 1.65 × 10−8 (1) |
| pAKOf | DR12 | 1.54 × 10−7 (0.4) | 3.34 × 10−6 (7.4) | 6.56 × 10−7 (1.5) | 4.52 × 10−7 (1) |
| p4bpRep | DR12 | 1.67 × 10−7 (0.9) | 7.94 × 10−7 (4.1) | 2.14 × 10−7 (1.4) | 1.93 × 10−7 (1) |
| pBENDx | DR12 | 5.45 × 10−7 (0.9) | 1.04 × 10−6 (1.6) | 3.94 × 10−7 (0.6) | 6.32 × 10−7 (1) |
| pRIP DSx | DR12 | 4.95 × 10−8 (0.6) | 2.88 × 10−7 (3.5) | 1.57 × 10−7 (1.9) | 8.26 × 10−8 (1) |
| pLamRep | DR12 | 1.80 × 10−8 (0.4) | 1.14 × 10−5 (268.2) | 1.59 × 10−6 (37.4) | 4.25 × 10−8 (1) |
| pMCD | HCT | 2.43 × 10−8 (0.2) | 4.80 × 10−6 (39.3) | 1.36 × 10−6 (11.2) | 1.22 × 10−7 (1) |
| pMCD | HeLa | 1.36 × 10−8 (1.8) | 2.93 × 10−7 (39.1) | 1.42 × 10−7 (19.0) | 7.49 × 10−9 (1) |
| pMCDΔAT | HeLa | 3.23 × 10−8 (0.8) | 1.47 × 10−7 (3.8) | 2.17 × 10−7 (5.7) | 3.82 × 10−8 (1) |
| pATrep | HeLa | 3.81 × 10−8 (0.3) | 8.82 × 10−7 (5.8) | 2.13 × 10−7 (1.4) | 1.50 × 10−7 (1) |
| pLamRep | HeLa | 2.91 × 10−8 (0.5) | 8.86 × 10−7 (16.1) | 3.73 × 10−7 (6.8) | 5.47 × 10−8 (1) |
a
Values in the table are from a typical experiment out of at least three separate transfection experiments for each construct.
b
All DNA was isolated from pools of cells stably transfected with the mutant construct listed.
c
All DNA constructs were stably transfected into either DR12, HeLa, or HCT116 (HCT) cells.
d
Abundance was calculated as described in Materials and Methods.
e
Initiation activity, in parentheses, was calculated as described in Materials and Methods.
f
Previously published results (5).