FIG. 7.

Role of Nox1 in EGF-induced ROS generation. (A) Caco-2 cells were electroporated with either pFLAG-βPix or the empty vector together with pSUPER-Nox1. After being cultured for 48 h, the cells were deprived of serum for 12 h. After EGF (100 ng/ml) treatment, the generation of H₂O₂ was measured on the basis of DCF fluorescence. Data are means ± SE of values from three independent experiments. (B) Total cell lysates of each sample from panel A were prepared and subjected to immunoblot analysis with antibodies to FLAG; the filter was then reprobed with antibodies to β-actin. (C) Total RNA was prepared from each sample from panel A. An RT-PCR demonstrates Nox1 expression. GAPDH served as a loading control.