FIG. 1.

Restriction and domain map of KlCat8p and its deletion alleles. The two black boxes represent the zinc cluster and the downstream coiled-coil domains, highly conserved in more than 100 zinc cluster proteins. The grey line represents the middle homology region found in more than 50 zinc cluster proteins. The small black squares cover regions that are highly conserved only among the three known yeast Cat8p homologues. The stars indicate the positions of the eight motifs conserved in zinc cluster proteins as described by Poch (25). Serines 661 and 871 are positioned on the KlCat8p sequence. Grey arrows indicate the sites used in the internal deletion Δ48-3405, and black arrows point to the C-terminal deletion Δ3204-4335.