TABLE 3.
Carbon source regulation of transactivation properties of serine mutant alleles of Kl-Cat8p and ScCat8p fusion proteinsa
| Allele | 2% glucose | 0.05% glucose | 2% galactose-1% raffinose | 3% glycerol | 3% ethanol |
|---|---|---|---|---|---|
| Gal4 | 0.03 (0.0) | 0.06 (0.01) | 0.08 (0.01) | 0.0 (0.0) | 0.0 (0.0) |
| Gal4 KlCat8 | 0.06 (0.03) | 0.14 (0.06) | 0.95 (0.4) | 2.6 (0.8) | 36 (7.9) |
| Gal4 KlCat8S661A | 0.01 (0.0) | 0.0 (0.0) | 0.03 (0.05) | 0.66 (0.4) | 13 (1.1) |
| Gal4 KlCat8S661E | 0.54 (0.16) | 10 (0.67) | 14 (4.1) | 23 (0.6) | 25 (0.44) |
| Gal4 KlCat8S871A | 0.08 (0.03) | 0.29 (0.14) | 1.2 (0.03) | 2.5 (0.25) | 49.1 (2.8) |
| Gal4 KlCat8S871E | 0.07 (0.02) | 0.38 (0.11) | 2.2 (0.15) | 5.0 (2.5) | 36 (7.9) |
| Gal4 ScCat8 | 0.25 (0.04) | 0.25 (0.02) | 1.4 (0.07) | 10.5 (0.4) | 43.14 (2.9) |
| Gal4 ScCat8S562A | 0.22 (0.2) | 0.2 (0.02) | 0.48 (0.12) | 3.92 (1.0) | 26.8 (0.82) |
| Gal4 ScCat8S562E | 1.02 (0.04) | 9.6 (0.7) | 6.8 (0.54) | 26 (2.2) | 44 (9.7) |
| Gal4 ScCat8S802-4A | 0.21 (0.24) | 0.28 (0.04) | NDb | ND | 51.2 (5.6) |
a
Strain MAV103 was transformed with pGBT9-based plasmids expressing different alleles of Gal4-KlCat8p and Gal4-ScCat8p fusions from a truncated ScADH1 promoter. Transformants were grown on selective culture medium supplemented with the indicated carbon sources and assayed for β-galactosidase activity as described in Materials and Methods. β-Galactosidase activities (Miller units) are means of three independent measurements, with standard deviations in parentheses.
b
ND, not determined.