FIG. 1.

Quantitative analysis of Fut4, Fut9, and β-actin transcripts in developing early embryos and PGC. (A) Electrophoretic patterns of each product by competitive RT-PCR. The standard control for the PCR was performed with a mixture of standard plasmid (Fut4, Fut9 [1.25 to 40 ag], β-actin [50 to 800 ag]) and a fixed concentration of each gene competitor DNA (Fut4, Fut9 [10 ag], β-actin [100 ag]). The coamplified DNA fragments derived from standard DNA or cDNA and from competitor DNA were subjected to electrophoresis in a 1% agarose gel and then visualized by ethidium bromide staining. (B) Bar chart showing expression levels of Fut4 and Fut9. Amounts of Fut4 and Fut9 transcripts were normalized by the amount of the β-actin transcript.