Table 1. Summary of PEG-like Nanoprobes (PN’s).
| PNDesignation | PeptideDesignation | Abs/em(nm) | MW,(kDa) Obs* | Volume FPLC Dia. (Fig. 2a)** | Quant. Yield(Fig. S3)*** | Cell NSB(Fig. S4)*** |
| Not Appl. | (DOTA)Lys-Cys | Not Appl. | 0.6356 | Not Appl. | Not Appl. | Not Appl. |
| Not Appl. | (DOTA)Lys-Cys(IR-783) | 789/807 | 1.3258 | Not Appl. | 0.053±0.008 | 95±1% |
| PN(783)3.0 | (DOTA)Lys(PEG 2 kDa)-Cys(IR-783) | 789/810 | 3.34 | 11.2 kDa 3.0 nm | 0.13±0.02 | 50±1% |
| PN(783)4.3 | (DOTA)Lys(PEG 5 kDa)-Cys(IR-783) | 789/810 | 6.29 | 35.2 kDa 4.3 nm | 0.16±0.03 | 20±1% |
| PN(783)6.1 | (DOTA)Lys(PEG 10 kDa)-Cys(IR-783) | 789/810 | 11.41 | 100.7 kDa 6.1 nm | 0.16±0.03 | 14±1% |
| PN(783)8.4 | (DOTA)Lys(PEG 20 kDa)-Cys(IR 783) | 789/810 | 21.28 | 262.5 kDa 8.4 nm | 0.16±0.04 | 14±1% |
| PN(783)10.0 | (DOTA)Lys(PEG 30 kDa)-Cys(IR-783) | 789/810 | 30.62 | 435.4 kDa 10.0 nm | 0.16±0.04 | 18±1% |
| PN(783)11.7 | (DOTA)Lys(PEG40 kDa)-Cys(IR-783) | 789/809 | 45.00 | 690 kDa 11.7 nm | 0.16±0.02 | 17±1% |
| Not Appl. | (DOTA)Lys-Cys(Cy3) | 544/559 | 1.2138 | Not Appl. | 0.32±0.07 | 98±1% |
| PN(545)4.3 | (DOTA)Lys(PEG 5 kDa)-Cys(Cy3) | 545/560 | 6.18 | 35 kDa | 0.59±0.09 | 31±1% |
| PN(545)10.0 | (DOTA)Lys(PEG 30 kDa)-Cys(Cy3) | 545/560 | 30.51 | 435 kDa | 0.61±0.10 | 15±1% |
| Not Appl. | (DOTA)Lys-Cys(Fl) | 497/521 | 1.0626 | Not Appl. | 0.50±0.11 | 0.4±0.1% |
| PN(497)4.3 | (DOTA)Lys(PEG 5 kDa)-Cys(Fl) | 497/521 | 6.04 | 35 kDa | 0.59±0.06 | 0.2±0.1% |
| PN(497)10.0 | (DOTA)Lys(PEG 30 kDa)-Cys(Fl) | 497/521 | 30.36 | 435 kDa | 0.60±0.08 | 0.1% ±0.1% |
*M.W. Obs. = molecular weights were determined from mass spectrometry results.
**Volumes are expressed as diameters in nm (to enable comparison with nanomaterials) and as the equivalent volumes of proteins (to enable comparison with proteins).
***Values are means±1 S.D.