Skip to main content
. 2014 Apr 29;9(4):e95957. doi: 10.1371/journal.pone.0095957

Figure 5. Over-expression of the MBNL1 constructs leads to robust protein and mRNA over-expression.

Figure 5

A. Western blot of the DM1 human myoblasts transfected with various EGFP-MBNL1 fusion constructs shows significant over-expression of the fusion proteins as compared to the endogenous MBNL1. # represents the Del-Linker fusion protein which cannot be picked up by the MBNL1 antibody. B. Real Time PCR was performed to quantify total MBNL1 levels in cells over-expressing the various EGFP-MBNL1 constructs and compared to the levels from mock transfected cells. There was extensive over-expression in these cell lines. * represents the Del-N constructs which the MBNL1 qRTPCR primers did not recognize due to their location within the deleted region.