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. 2014 Apr 29;9(4):e96177. doi: 10.1371/journal.pone.0096177

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© 2014 Chen et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

The cell migration and invasion capacity of pancreatic cancer cells treated with conditioned media (CM) from NFs, Ctrl-treated CAFs and QYHJ-treated CAFs was compared using transwell chambers with or without Matrigel coating. The numbers of cells that traveled through the membrane were counted in 10 fields under a ×20 objective lens. Original magnification, ×200. The results represent the means ± SD of the values obtained in three independent experiments. Statistical significance was calculated using ANOVA. * P<0.05.