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. 2014 Apr 29;9(4):e96376. doi: 10.1371/journal.pone.0096376

Figure 4. Independent verification of gene expression using qPCR.

Figure 4

Gene expression was analyzed on an independent set of RJF brains (females: n = 4, males: n = 7). A) The exonic region of the novel gene within MHM was more strongly expressed compared to EST region downstream and within intronic region. B) The ZFR paralog on chromosome 1 was highly up-regulated in females, while the ZFR paralog on chromosome Z showed an expected none dosage compensated male bias. Independent t-test adjusted for unequal variance, ***p<0.0001, *p<0.01, #p<0.1.