Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Interaction between PKR and endogenous TRAF proteins. (A) Extracts (50 μg) from HeLa cells left untreated or treated with IFN-α/β for 16 h were separated by SDS-PAGE and analyzed by immunoblotting (Western blotting [WB]) with anti-PKR or anti-TRAF2 antibodies. (B) Extracts (1 mg) were immunoprecipitated (IP) with antibodies against TRAF2, TRAF5, PKR, or FLAG and thoroughly washed, and immunocomplexes were analyzed by SDS-PAGE and subjected to immunoblotting with antiserum to PKR.
