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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Eur J Immunol. 2014 Feb 20;44(5):1517–1525. doi: 10.1002/eji.201344170

Figure 4.

Figure 4

CD16 expression is downregulated on expanded NK cells incubated with 721.221, but not with 1106mel cells. (A) Freshly isolated NK cells were stained for CD16 expression (input). (B) NK cells from (A) were incubated with 721.221 (top) and with 1106mel (bottom) for five and 16 h or for 7 days and the expression levels of CD16 were determined. (C) Levels of CD107a expression determined prior to incubation with target cells (input, left dot plots) or following 5-h incubation of the NK cells with 721.221 or with 1106mel. The numbers in the quadrants indicates percentages. Data shown are representative of three experiments performed. (D) CD16 levels in the supernatants of NK cells (incubated either with 721.221 cells or with 1106mel) were quantified by ELISA after 16 h. Data are shown as mean + SD from a single experiment representative of five performed. *p < 0.001, two tailed Student’s t-test.