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[3H]glycine uptake by BBMV. Vesicles were prepared in 1 mM MgSO4, 2 mM HEPES-Tris (pH 7.35), 50 mM K2SO4, and 250 mM mannitol. Vesicles were preincubated with valinomycin (10 mg/mg of protein). External medium contained 1 mM MgSO4, 2 mM HEPES-Tris, and either 100 mM NaCl, 200 mM mannitol, or 250 mM mannitol. Vesicles were incubated at 37°C in an uptake buffer containing 20 nM [3H]glycine.
