FIGURE 5:

Expression of Kalirin constructs lacking the GEF and PDZ-binding domains causes spine formation. Top, diagrams illustrating the proteins expressed. Cortical neurons were transfected with vector encoding GFP only (A) or doubly transfected with vectors encoding GFP and MycKal7 (B), MycΔKal7 (not shown), MycKalSolo (C), or MycKal4 (D) at the time of plating. Neurons were visualized for GFP (green) and Myc (red) at DIV18. Cortical neurons expressing MycKalSolo (E) or MycKal4 (F) were stained simultaneously with antibodies to Myc (red), Vglut1 (green), and MAP2 (blue); for both, most Vglut1 puncta were juxtaposed to Myc puncta.