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. 2004 May;24(10):4476–4486. doi: 10.1128/MCB.24.10.4476-4486.2004

FIG. 1.

FIG. 1.

The BAF complex is required for cellular responses to IFN-α and poly(I)/poly(C) stimulation. (A) BAF47 was efficiently knocked down by RNA interference. HeLa cells were transfected with a control vector (lane 1) or a siRNA construct targeting BAF47 (lane 2) and selected for 2 days in puromycin. The remaining cells were lysed and analyzed by Western blotting with antibodies against BAF47, BRG1, BRM, or β actin. (B) The BAF complex is required for the induction of IFN target genes by IFN-α. HeLa cells were transfected with the siRNA construct targeting BAF47 (siBAF47) and selected for 2 days with puromycin. Following stimulation with 500 U of IFN-α/ml for 8 h, the total RNAs were isolated for RT-PCR analysis with the primers indicated on the left sides of the panels. (C) The BAF complex is required for the induction of IFN target genes by poly(I)/poly(C). HeLa cells were transfected and selected as described above. Following stimulation with poly(I)/poly(C), total RNAs were isolated and analyzed as described above.