FIG. 4.
Expression of BRG1 results in more rapid kinetics and higher levels of the IFITM1 gene induction in SW-13 cells in response to IFN-α. (A) Analysis of IFITM1 mRNA expression induced by treatment with IFN-α. Total RNAs extracted from HeLa cells and SW-13 cells treated with IFN-α were reverse transcribed, amplified by PCR with IFITM1 primers, slot blotted onto nylon membrane, and detected by hybridization with a 32P-labeled IFITM1 cDNA probe. (B) The slot blot was quantified by PhosphorImager analysis and plotted after normalization to β actin signals. (C) The same samples were amplified with ISG15 primers and analyzed as described above. (D) SW-13 cells were transfected with pBJ5 or pBJ5-BRG1 for 24 h, followed by treatment with 500 U of IFN-α/ml for various times. The total RNAs were analyzed as described for panel A. (E) Quantification of the samples in panel D by PhosphorImager analysis.