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. 2014 May 1;25(9):1523–1531. doi: 10.1091/mbc.E13-10-0590

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© 2014 Ishaq et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — TNF signaling involved in the activation of ASK1, p38, and JNK by AGP. (a–c) Mel007 cancer cells were treated with AGP with or without pretreatment with TNFR1- neutralizing antibody or NAC. Western blot analysis was performed on cell lysate using different antibodies as indicated (p-ASK1, S83 phosphospecific antibody for ASK1; p-JNK, phosphospecific antibody for JNK; p-p38, phosphospecific antibody for p-38). GAPDH antibody was used as a loading control. (d) The basal protein expression levels for ASK1, p38, JNK, ASK1-phos, p38-phos, and JNK-phos were quantified in normal melanocytes and Mel007 cells by Western blot analysis. GAPDH antibody was used as a loading control.