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. 2014 May 1;25(9):1532–1542. doi: 10.1091/mbc.E13-10-0600

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© 2014 Miyamoto et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Rab35 is required for FBD-102b cell differentiation. (A) Cells were transfected with an siRNA for control (luciferase), Rab35#1, or Rab35#2 and immunoblotted with an antibody against Rab35, MBP, or β-actin. (B) The bands of each Rab35 protein were semiquantified (n = 5). (C) The bands of each MBP protein were semiquantified (n = 5). (D) Cells transfected with an siRNA for control, Rab35#1, or Rab35#2 were allowed to differentiate for 3 d. Representative images are shown. Scale bar: 20 μm. (E) The percentage of cells in stage 3 was determined (n = 10 fields in three experiments). (F) Cells transfected with an siRNA for control or Rab35#2 as well as control vector or GFP-siRNA–resistant Rab35 were allowed to differentiate for 3 d. The percentage of cells in stage 3 was determined (n = 10 fields in three experiments). Data were evaluated with one-way ANOVA (*, p < 0.01; **, p < 0.05).