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. 2014 May 1;25(9):1532–1542. doi: 10.1091/mbc.E13-10-0600

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© 2014 Miyamoto et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Arf6 is required for FBD-102b cell differentiation. (A) Cells were transfected with either an siRNA for control, or Arf6, and immunoblotted with an antibody against Arf6, MBP, or β-actin. (B) The bands of each Arf6 protein were semiquantified (n = 3). (C) The bands of each MBP protein were semiquantified (n = 3). (D) Cells transfected with an siRNA for control or Arf6 were allowed to differentiate for 3 d. Representative images are shown. Scale bar: 20 μm. (E) The percentage of cells in stage 3 was determined (n = 10 fields in three experiments). (F) Cells transfected with an siRNA for control or Arf6 as well as control vector or ZsGreen-siRNA–resistant Arf6 were allowed to differentiate for 3 d. The percentage of cells in stage 3 was determined (n = 10 fields in three experiments). Data were evaluated with Student's t test (*, p < 0.01).