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. 2014 May 1;25(9):1532–1542. doi: 10.1091/mbc.E13-10-0600

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© 2014 Miyamoto et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Arf6 is activated following differentiation in FBD-102b cells. (A) Cells were allowed to differentiate for 0–3 d and lysed to be used in an affinity precipitation of active GTP-bound Arf6 with recombinant GST-GGA3-ABD. Immunoblots for total Arf6 and β-actin are also shown. (B) Bands corresponding to each GTP-bound Arf6 protein were scanned and semiquantified (n = 4). Data were evaluated with one-way ANOVA (*, p < 0.01).