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. 2014 May 1;25(9):1532–1542. doi: 10.1091/mbc.E13-10-0600

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© 2014 Miyamoto et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Rab35/ACAP2/Arf6 is required for myelin formation. (A) Primary OPCs infected with an shRNA for control, Rab35, ACAP2, or Arf6 were cocultured with DRG neurons for 4 wk and stained with an anti-MBP antibody (red) and DAPI (blue). Representative images are shown. Scale bar: 50 μm. (B) The percentages of the MBP-positive myelin segments were determined (n = 7 fields in three experiments). Data were evaluated with one-way ANOVA (*, p < 0.01). (C–E) Coculture lysates were immunoblotted with an antibody against MBP or β-actin.